gel image analyzer chemdoc xrs (Bio-Rad)
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gel image analyzer chemdoc xrs
Gel Image Analyzer Chemdoc Xrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemdoc+image+analyzer/chemdoc+xrs/pmc10970026-141-27-30
Average 90 stars, based on 1 article reviews
Gel Image Analyzer Chemdoc Xrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemdoc+image+analyzer/chemdoc+xrs/pmc10970026-141-27-30
Average 90 stars, based on 1 article reviews
gel image analyzer chemdoc xrs - by Bioz Stars,
2026-09
90/100 stars
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Software:Article Title: Structural modeling defines transmembrane residues in ADAM17 that are crucial for Rhbdf2–ADAM17-dependent proteolysis Article Snippet: After blocking in 5% non-fat milk at room temperature for 1 h, the membranes were incubated in primary antibody generated against the cytoplasmic domain of ADAM17 overnight at 4°C (1:500 dilution, for validation of specificity using Adam17 −/− MEFs, please see McIlwain et al., 2012 ), washed three times in Tris-buffered saline (TBS) with 0.5% Tween 20 and then incubated in 1:1000 horseradish peroxidase (HRP)-labeled donkey anti-rabbit-IgG secondary antibody. .. Bound antibodies were detected using the ECL system (Amersham Biosciences, Waltham, MA) and a Article Title: ADAM17 stabilizes its interacting partner inactive Rhomboid 2 (iRhom2) but not inactive Rhomboid 1 (iRhom1) Article Snippet: The nitrocellulose membranes were then washed three times in PBS, 0.05% Tween 20 and then incubated in either HRP-labeled goat anti-rabbit secondary antibody, HRP-labeled goat anti-mouse secondary antibody (1:5000; Promega, Madison, WI), or HRP-labeled goat anti-rat secondary antibody (1:5000; Sigma). .. Bound antibodies were detected using the ECL system (Thermo Fisher Scientific) and a Incubation:Article Title: iRhom2 controls the substrate selectivity of stimulated ADAM17-dependent ectodomain shedding Article Snippet: Comparable amounts of protein were separated on 10% (wt/vol) SDS/polyacrylamide gels and transferred onto poly(vinylidene difluoride) membranes (BioTrace; Pall Corporation). .. These were blocked with 3% (wt/vol) skim milk in TBS and then incubated with primary antibodies and washed in 0.1% Tween-TBS, and bound primary antibodies were detected with peroxidase-conjugated goat anti-mouse or goat anti-rabbit antibodies (Promega) using the ECL detection system (Amersham Biosciences) and a Article Title: Identification of Molecular Determinants in iRhoms1 and 2 That Contribute to the Substrate Selectivity of Stimulated ADAM17 Article Snippet: .. Then the membranes were washed 3 times in 0.05% Tween-20 in PBS, and bound antibodies were detected following incubation with ECL buffers (Amersham Biosciences, Amersham, UK) and imaged with a |